UV‐induced DNA damage disrupts the coordination between replication initiation, elongation and completion

Replication initiation, elongation and completion are tightly coordinated to ensure that all sequences replicate precisely once each generation. UV-induced DNA damage disrupts replication delays elongation, which may compromise this coordination leading genome instability cell death. Here, we profiled the Escherichia coli as it recovers from UV irradiation determine how these replicational processes respond. We show oriC initiations continue occur, copy number enrichments in region. At late times, combination of new delayed elongating forks converging terminus appear stress or impair reaction, a transient over-replication region chromosome. In mutants impaired for restoring including recA, recF uvrA, degrades remains static, suggesting death occurs early after is disrupted, leaving partially duplicated genomes. completing replication, recBC, sbcCD xonA recG, recovery initiation leads bottleneck, where nonterminus amplified accumulates, indicating mutants, likely resulting mis-segregation unbalanced unresolved chromosomes when cells divide.